Transfection of Nerve Cells

摘要

Transfection is a method of transforming cells based on the introduction into living cells of plasmidsencoding a particular protein or RNA. This review describes the main methods of transfection and con-siders their advantages and disadvantages. Most attention is paid to lentivirus transduction as one of themost efficient methods for transforming nerve cells. The development of current transfection systemsbased on lentivirus vectors is described and a brief review of studies performed using in vivo and in vitrolentivirus transfection of nerve cells is presented.