Quantitative maps of protein phosphorylation sitesacross 14 different rat organs and tissues

摘要

Deregulated cellular signalling is a common hallmark of disease, and delineating tissuephosphoproteomes is key to unravelling the underlying mechanisms. Here we present thebroadest tissue catalogue of phosphoproteins to date, covering 31,480 phosphorylation siteson 7,280 proteins quantified across 14 rat organs and tissues. We provide the data set as aneasily accessible resource via a web-based database, the CPR PTm Resource. A major fractionof the presented phosphorylation sites are tissue-specific and modulate protein interactionnetworks that are essential for the function of individual organs. For skeletal muscle, we findthat phosphotyrosines are over-represented, which is mainly due to proteins involved inglycogenolysis and muscle contraction, a finding we validate in human skeletal muscle biopsies.Tyrosine phosphorylation is involved in both skeletal and cardiac muscle contraction, whereasglycogenolytic enzymes are tyrosine phosphorylated in skeletal muscle but not in the liver. Thepresented phosphoproteomic method is simple and rapid, making it applicable for screening ofdiseased tissue samples.