RNA detection has become an integral part of current biomedical research. Up to now, thereverse transcription–PCR has been the most practical method to detect mRNA targets.However, RNA detection by reverse transcription–PCR requires sophisticated equipment andit is highly sensitive to contamination with genomic DNA. Here we report a new isothermalreaction to simultaneously amplify and detect RNA, based on cleavage by DNAzyme andsignal amplification. Cleavage-based signal amplification of RNA cannot be contaminated bygenomic DNA and is suitable for the detection of both mRNA and microRNA targets, withhigh specificity and sensitivity. Moreover, the detection results can be reported in a colorimetricor real-time fluorometric way for different detection purposes.
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