Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9

摘要

Elucidation and manipulation of human, animal and plant genomes is key to basic biology research, medical advances and crop improvement. The development of targeted genome editing, particularly homologous recombination-based gene replacement, is of great value in all organisms. Recent advances in engineered nucleases with programmable DNA-binding specificities, such as zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), have provided valuable means to create targeted mutations in metazoan and plant genomes with high specificity1-6.