CRISPR/Cas9介导基因组编辑技术在植物基因中的研究进展

摘要

CRISPR/Cas9-mediated genomic editing could generate double stranded breaks in special sites,which could be repaired by homologous recombination or non-homologous end joining,and it resulted in base deletion or insertion in genome.The advantages of the CRISPR/Cas9 are simple operation and high effi-ciency of mutation.The article describes the basic structure and classification of the CRISPR/Cas9 and its de-velopment and progress in plants.%CRISPR/Cas9介导的基因组编辑技术是在DNA双链的特定位置形成双链断裂,然后通过同源重组或非同源末端连接方式进行修复,造成基因组碱基局部缺失或插入而引起基因突变,它具有操作简单、突变效率高等优势.笔者归纳了CRISPR/Cas9系统的基本结构、分类及其在植物基因中的研究进展和未来的发展方向.