PCR amplification, cloning and In-silico characterization of promoter regions of HMW-glutenin, alpha/beta-gliadin and triticin genes from wheat variety K-68

摘要

HMW-glutenin and alpha/beta-gliadin promoter regions were amplified through PCR from wheat variety K-68 using primers designed on the basis of published nucleotide sequences of HMW-glutenin and alpha/beta-gliadin genes of wheat variety Chinese Spring.In addition, an 821 bp promoter'region of triticin gene was also amplified and cloned from K-68, using genome walking strategy. Nucleotide sequences of the cloned promoter regions were analyzed using promoter analysis softwares like PLACE and Promoter Scan which revealed the presence of several cw-acting regulatory motifs required for driving endosperm specific expression of the genes. Several promoter elements, having diverse functions, were also identified in the cloned promoter regions.