Antimicrobial and antioxidant activity of protamine prepared from salmon sperm

摘要

Protamine-strong basic protein was prepared from salmon(chum salmon, Oncorhynchus keta sperm by several pretreatment method. And there were determined yield, amino acid composition, antimicrobial and antioxidant activity of protamine on each pretreatment condition. The yield of protamine was different according to pretreatment, and ultrasonicating, homogenizing and microwaving pretreatment were about 16.0 percent, 15.5 percent and 10 percent, respectively. The main amino acid of P60(microwaving pretreatment for 10 min at 80 deg C) and UU6(ultrasonicating pretreatment for 60 min at 20 deg C) were arginine, proline and tryptophan, and arginine content of P60 and UU6 were 61 percent, 53 percent, respectively. On the other hand, main amino acid of M(homogenizing pretreatment by mixer) were methionine, proline and arginine, the content were 34 percent, 28 percent and 11 percent, respectively. Also MC(homogenizing pretreatment with H_2SO_4 soln. by mixer) was very different with P60, UU6 and M, the content of MC were proline 44.8 percent and arginine 39.7 percent. Prepared protamines showed antimicrobial activity to several gram(+) and gram(-) strain. In particular, the UU6 and P60 protamine has strong antimicrobial activity to Bacillus subtilis and Escherichia coli, and the activity was increased with concentration increasing. Regardless of pretreatment method, all protamine showed antioxidant activity and the EDA_(50) of P60, UU6, M and MC were 101 #mu#g/mL, 410 #mu#g/mL, 523 #mu#g/mL and 490 #mu#g/mL, respectively.