Optimization of extraction conditions from Hericium erinaceus by response surface methodology

摘要

Response surface methodology (RSM) was used to monitor the characteristics of ethanol extracts from Hericium erinaceus. A central composite design was applied to investigate the effects of independent variables, ethanol concentration (X_1), and sample ratio (X_2) on dependent variables, soluble solid (Y_1), total phenols (Y_2), crude protein (Y_3), electron donating ability (Y_4), and browning color (Y_5) of the extracts. As the sample ratio increased, the soluble solid content increased. Ethanol concentration played a minor role. Total phenols and crude protein increased with sample ratio. Sample ratio had a greater effect than alcohol concentration in the extraction of soluble solid, total phenols, crude protein, and browning color, with an exception of electron donating ability. The optimum ranges at 2 hr extraction was 3.9 approx 5.0 g/100 mL in sample ratio and 36 approx 52 percent in ethanol concentration. Predicted values at the optimized conditions were acceptable when compared to experimental values.