Inhibitory Effect of Rumex Crispus L. Fraction on Adipocyte Differentiation in 3T3-L1 Cells

摘要

The anti-obesity effect of ethanol extract and their fractions from Rumex Crispus L. on the differentiation of 3T3-L1 pre-adipocytes to adipocytes was investigated by suppressing adipocyte differentiation and lipid accumulation with Oil red O assay, western blot and real-time PCR analysis. Ethyl acetate fraction of Rumex crispus L. significantly inhibited adipocyte differentiation when treated during the adipocyte differentiation process, as assessed by measuring fat accumulation using Oil red O staining. In inducing differentiation of 3T3-L1 preadipocytes in the presence of an adipogenic cocktail, isobutylmethylxanthine (IBMX), dexamethasone- and insulin- along with ethyl acetate fraction residue processing treatment significantly decreased proteinexpression of obesity-related proteins, such as peroxisome-proliferators-activated-receptor-y (PPARy) and CCAAT enhancer-binding-proteins a (C/EBPa). These results indicate that ethyl acetate fraction of Rumex crispus L. is the most effective candidatefor preventing obesity. However further studies will be needed to identify the active compounds that confer the anti-obesity activity of ethyl acetate fraction from Rumex crispus L.