Immobilization of agarase for the agarooligosaccharide production

摘要

The condition for immobilization of the partially purified agarase from Bacillus cereus ASK202 and the properties of the immobilized enzyme have been investigated. Agarase was immobilized on various supports by entrapment method. The enzyme immobilized on Na-alginate bead showed the highest activity among those studied. The optimal reaction conditions of the immobilized agarase were obtained in 3%(w/v) Na-alginate for the matrix, bead diameter of 2.5 mm, 1 unit of agarase solution and 1.0% (w/v) calcium chloride solution. The optimum aH and temperature of the immobilized agarase were aH 7.0 and 40 deg C, respectively. K_m and V_(max) values were 0.5 mg/ml and 4.4 mg/ml centre dot min, respectively. The immobilized agarase converted agar to agarobiose and agarotetraose, and their total conversion ratio under the optimal condition was 89%.