Purification of fungal protease produced by Mucor racemosus f. racemosus PDA 103 form korean traditional Meju

摘要

The protease produced by Mucor racemosus f. racemosus PDA 103 from meju was purified by precipitating with 80% saturated ammonium sulfate, CM Sephadex C-50 ion-exchange chromatography, and secondary Sephadex G-100 gel filtration chromatography. The specific activity of the purified enzyme was 60.1 unit/mig protein and the purification told of the enzyme was 83.5. The molecular weight of the enzyme was estimated 33,746 Da and the enzyme was elucidated as monomer by LC-MS and SDS-PAGE. The number of amino acids was evaluated about 330 residues.