Antifouling Activity of Giffinisterone B and Oleamide Isolated from a Filamentous Bacterium Leucothrix mucor Culture against Ulva pertusa

摘要

The filamentous bacterium Leucothrix mucor, an epiphyte of seaweed, showed antifouling activity against Ulva pertusa spore settlement and germling development. The chemical constituents representing the antifouling activity were identified as giffinisterone B and oleamide based on nuclear magnetic resonance (NMR) spectroscopy and mass spectroscopy (MS). Approximately 3.6 mg of giffinisterone B and 2.8 mg of oleamide were isolated from 1.6 g of Leucothrix mucor crude extract. Giffinisterone B fully inhibited spore settlement and germling development at 100 mug/mL. Oleamide inhibited spore settlement at 10 mug/mL and germling development at 100 mug/mL.