Isolate specific detection of mycorrhizal fungi using genome specific primer pairs.

摘要

Random amplification of polymorphic DNA analysis was used to generate DNA fragments unique to isolates of 2 arbuscular mycorrhizal fungi, Glomus mosseae and Gigaspora margarita. Sequence analysis of these fragments allowed generation of primer pairsand subsequent specific identification of these genomes even in the presence of competing genomic DNA's. It is suggested that this approach could be used to specifically detect the presence of these fungi in mixtures of spores or infected roots.