Comparison of Two Kinds of Methods to Determine the Titer of Recombinant Retrovirus Containing #beta#-Globin Gene Based on G418 Selection

摘要

Four recombinant retrovirus (RV) vectors containing human #beta#-globin gene and regulatory elements were constructed. To determine the titers of recombinatn RV from corresponding producer cell lines, we compared two kinds of method (the simple and the conventional) based on G418 resistance,which is conferred by neo gene of RV vector. The results demonstrated that the simple method shortened the selection period to 3 d instead of 10-12 d with the conventional method and reduced the amount of work; importantly, the titers determined by the simple method were not different signficanty from those measured by the conventional method. It can be concluded that the simpel method can be used to determine the titers of recombinant RV containing not only cDNA but also genomic DNA with introns and complex regulatory elements instead of the conventional method.