Genetic diversity analysis and DNA fingerprinting of elite chilli pepper lines using SSR markers.

摘要

Hybrid cultivars of chilli pepper (Capsicum annuum L.) have become very popular due to their superior performance. Punjab Agricultural University, Ludhiana, India, developed a genetic male sterile (GMS) line 'MS-12' by introducing a nuclear male sterility gene ms509 (redesignated as ms10) from a French introduction. Subsequently, 'MS-12' has successfully been used to develop commercial F₁ hybrids. However, 'MS-12' has outlived its utility and there is a need to diversify the maternal parent. The investigation was carried out to identify the most divergent genotype from 'MS-12' among the six elite lines of chilli pepper by employing 58 SSR markers. Thirty produced polymorphic bands, revealing a total of 83 alleles with an average of 2.67 alleles per locus. Polymorphic information content (PIC) varied from 0.21 to 0.84, with an average value of 0.51. Based on PIC values and number of alleles amplified, the primer CAMS-072 was the most informative, followed by CAMS-311 and Hpms CaSIG-19. Lines under evaluation had more than 70% similar fragments. Dice similarity coefficients between any two genotypes varied from 0.70 to 0.95, with the lowest between 'PBC-535' with 'VR-16' and the highest between 'MS-12' sterile with 'MS-12' fertile. 'SL-412' and 'DCL-524' had a similarity coefficient of 0.93. Among the six elite lines assayed, 'PBC-535' was the most diverse from 'MS-12', followed by 'VR-16' with, similarity coefficients of 0.71 and 0.75, respectively. Some fragments were unique and can be used as cultivar specific markers for rapid discrimination. The discriminating power of these markers will be helpful in testing purity of F₁ hybrids and for registering elite germplasm.