Identification of antihypertensive peptides from peptic digest of twomicroalgae, Chlorella vulgaris and Spirulina platensis

摘要

The peptidic fractions that inhibited angiotensin 1-converting enzyme (ACE) were separated from the peptic digests of 2 microalgae, Chlorella vulgaris and Spirulina platensis, by ion exchange chromatography and gel filtration. Oral administration of peptidic fractions into spontaneously hypertensive rats at 200 mg/kg of body weight resulted in marked antihypertensive effects. Further separation of the peptidic fractions by ODS high-performance liquid chromatography furnished the following active peptides: Ile-Val-Val-Glu (inhibitory against ACE with an IC50 of 315.3 muM), Ala-Phe-Leu (63.8 muM), Phe-Ala-Leu (26.3 muM), Ala-Glu-Leu (57.1 muM), and Val-Val-Pro-Pro-Ala (79.5 muM) from C. vulgaris, Ile-Ala-Glu (34.7 muM), Phe-Ala-Leu, Ala-Glu-Leu, Ile-Ala-Pro-Gly (11.4 muM), and Val-Ala-Phe (35.8 muM) from S. platensis.