Effect of gamma irradiations on pineapple pollen germination and tube growth.

摘要

Preliminary studies were undertaken on the effects of irradiation on pollen germination in pineapple (Ananas comosus) to examine its potential for haploid plant production. Pollen of 2 varieties, Serrana (Smooth Cayenne) and Red Spanish, were subjected to gamma-irradiation from a 60Co source at 16 Gy/min (dose rates from 0 to 6 kGy). Pollen in vitro viability and tube length of both varieties decreased at the highest doses, and at 6 kGy there was no pollen germination recorded. Serrana (Smooth Cayenne) was more radiosensitive than Red Spanish. There were inverse correlations between irradiation dose and both pollen viability and tube length.