TRANSFORMATION OF HAPLOID AND DIPLOID POPLAR WITH DIFFER ENT GENE CONSTRUCTS AND ANALYSIS OF TRANSGENIC PLANTS

摘要

In previous experiments we have transferred a series of different gene constructs to various poplar genotypes. Transgenic lines were analysed in respect to T-DNA integration and T-DNA flanking genomic regions. Transgenic aspen carrying the Ac transposon from maize were also obtained. In these lines the excision of the Ac element as well as its re-integration was studied. The new positions of Ac in the genome were identified and flanking genomic regions sequenced. Interestingly, about one third of thesequences obtained show high homology to known genes from databanks. A similar study was undertaken for the genomic sequences flanking the T-DNAs. The results show a lower frequency of T-DNA tagged genes compared to Ac tagging. To apply the technique oftransposon tagging to Populus the problems of dioecy and long generation cycles are major deterrent. One possible solution is the establishment of haploid Populus lines and transfer of transposons in these lines. Initial experiments on the production and establishment of haploid Populus lines are reported.