TWO INSECT-RESISTANT GENES WERE TRANSFERRED INTO POPLAR HYBRID AND TRANSGENIC POPLAR SHEW INSECT-RESISTANCE

摘要

Modified Bt CryIA gene and cowpea trypsin inhibitor (CpTI) gene were co-integrated into the genome of clone NL-80106, P. deltoides Marsh. X P. simonii Carr. by co-infection with Agrobacterium tumefaciens LBA4404. PCR and PCR-Southern blotting analysis showed that both Bt CrylA and CpTI genes were integrated into the transgenic poplar. Insect bioassay indicated that two-gene transgenic poplar were significantly resistant to the larvae of Lymantria dispar, whereas the untransformed control plants were sensitive to them. It caused a decreased leaf consumption by larvae, a lower larval weight and a higher larval motility . ELIS A analysis of the transgenic clones proved that Bt and CpTI genes were expressed . Bt and CpTI transgenic poplar plants can be used prospectively as the material for selecting highly and long-term insect-resistant poplar clones in the future.