Development of a real-time PCR assay to control the illegal trade of meat from protected capercaillie species (Tetrao urogallus).

摘要

A rapid and highly species-specific real-time polymerase chain reaction (PCR) assay has been developed for the detection of capercaillie DNA (Tetrao urogallus) in meat and meat mixtures. The method combines the use of capercaillie-specific primers, that amplify a 142bp fragment of the mitochondrial 12S rRNA gene, and a positive control primer pair that amplifies a 141bp fragment of the nuclear 18S rRNA gene from eukaryotic DNA. SYBR((R)) Green dye or TaqMan((R)) fluorogenic probes were used to monitor the amplification of the target genes. Results obtained with the use of TaqMan((R)) probes as detection platform increased the specificity of the real-time PCR assay in comparison with the results obtained using SYBR((R)) Green. The proposed real-time PCR assay represents a rapid and straightforward method for the accurate identification of capercaillie that could be used by law enforcement agencies as a tool for the control of poaching and illegal trade of meat from this protected species.