An anti-diabetic TCM formula consisting of Schizandra chinensis (SC), Coptis chinensis (CC), guava leaves (PG) and mulberry leaves (MA) was developed based on its alpha-glucosidase, DPP-4 and AGE inhibitory activities in vitro using response surface methodology (RSM). An in vivo study was then carried out to confirm the anti-diabetic function of the formula. RSM results showed that the optimum anti-diabetic TCM formula was SC (3000 mug ml--1), CC (80 mug ml--1), PG (374.56 mug ml--1) and MA (480 mug ml--1). For the in vivo study, insulin resistant mice were induced by high-fat/high-sucrose (HF/HS) feeding for 6 wk. Administration of the developed formula markedly decreased non-fasting blood glucose in HF/HS diet mice. Also, the formula decreased blood glucose levels in the insulin tolerance test. Results suggest that the anti-diabetic mechanism of the formula may be due to decreased insulin resistance. The blood fructosamine level in the high dose group was markedly lower than the HF/HS and normal control groups, indicating that the formula could improve middle term glucose levels and reduce the risk of complications. Contents of berberine and 1-deoxynojirimycin in the formula were 4.7 +or- 0.4 and 77.1 +or- 1.1 mug ml--1, respectively. These 2 compounds can be used as indicators for quality control during production.
展开▼
机译：根据其在体外的α-葡萄糖苷酶，DPP-4和AGE抑制活性，开发了由五味子（SC），黄连（CC），番石榴叶（PG）和桑叶（MA）组成的抗糖尿病中药配方响应面方法（RSM）。然后进行体内研究以确认该配方的抗糖尿病功能。 RSM结果表明，最佳的抗糖尿病中药配方为：SC（3000毫升/ ml -1 ），CC（80毫升/ ml -1 ），PG（374.56毫升） ml -1 ）和MA（480杯ml -1 ）。对于体内研究，通过高脂/高蔗糖（HF / HS）喂养6周来诱导胰岛素抵抗小鼠。在HF / HS饮食小鼠中，使用已开发的配方可显着降低非空腹血糖。同样，该配方在胰岛素耐受性测试中降低了血糖水平。结果表明该配方的抗糖尿病机制可能是由于胰岛素抵抗降低所致。高剂量组的血果糖胺水平明显低于HF / HS组和正常对照组，表明该配方可以改善中期葡萄糖水平并降低并发症的风险。该配方中的小ber碱和1-脱氧野rim霉素的含量分别为4.7 +或-0.4和77.1 +或-1.1杯ml -1 。这两种化合物可用作生产过程中质量控制的指标。
展开▼
