Ultrasonically assisted extraction of rutin from Artemisia selengensis Turcz: comparison with conventional extraction techniques.

摘要

Ultrasonically assisted extraction (UAE) followed by high performance liquid chromatography (HPLC) analysis method for the fast extraction and determination of rutin in Artemisia selengensis Turcz has been developed. Artemisia selengensis Turcz has been used as food and herbal medicine for thousands of years in China. Rutin is one of the main active ingredients of this plant. The extraction of rutin from Artemisia selengensis Turcz was investigated by UAE. Special emphasis has been given to optimize the extraction conditions which were those with 90:10 (v/v) methanol-ethanol as solvent, 30:1 liquid-solid ratio, and 40 min extraction time. In order to show the superiority of UAE, other extractions were investigated, including microwave-assisted extraction, reflux extraction, and marinated extraction. The results showed that UAE was most suitable for the extraction of rutin in Artemisia selengensis Turcz because of its high extraction efficiency. Reversed phase-HPLC with ultraviolet detection was employed for the analysis of rutin in Artemisia selengensis Turcz. Under the optimum conditions, the calibration curve for the analyte was linear in the range of 0.34-20.7 micro g mL-1. The mean recovery of rutin was 100.77%, and its relative standard deviation was 0.37% (n=5). Three kinds of Artemisia selengensis Turcz from different habitats were investigated. The total content of rutin was 9.90, 6.23, 5.56 mg g-1, respectively.