Alternatives to Mammalian Expression: Bacterial, Yeast, and Cell-Free Systems Are Among Options Attempting to Replace Perennial Favorite

摘要

As the market for recombinant proteins continues to skyrocket, biotechs are exploiting a range of options to deal with multiple possibilities. At last month's CHI "Peptalk" conference, participants explored advances in a num-ber of different expression systems, including bacteria, yeast, mammalian cells, and even cell-free systems. A widely used protein-expression system is Pichia pastoris. A number of companies that provide molecular reagents also make plasmids and vectors for manipulating Pichia.