Mammalian In Vitro Translation (Cell-Free) Systems for Rapid and High-Yield Expression of Functional Proteins Including Antibodies

摘要

In vitro translation (IVT) using mammalian cell extracts is an alternative method to in vivo mammalian protein expression. Using two different mammalian cell lines (HeLa and CHO) we prepared highly translation-competent extracts for expressing a variety of genes from mammals, bacteria and protozoa. The Thermo ScientificT 1-Step Human High-Yield and CHO High-Yield IVT systems provide protein expression through the addition of a DNA of interest to a mixture of HeLa or CHO cell lysate and reaction mix followed by incubation for 1-6 hours at 30°C.