Liquid Chromatography-Tandem Mass Spectrometric Determination of Geniposide in Rat Plasma and its Pharmacokinetic Application

摘要

Geniposide is a biologically active ingredient of gardenia fruit. A liquid chromatography-tandem mass spectrometric method was developed and validated for the determination of geniposide in rat plasma. The plasma samples were pretreated by solid-phase extraction and introduced into a BDS Hypersil C_(18) column (50 × 2.1 mm, 5 μm) for chromatographic separation. The mobile phase consisted of 0.1% formic acid and 0.1% formic acid in acetonitrile, and gradient elution was performed at a flow rate of 0.25 mL/min. For mass spectrometric detection, multiple reaction monitoring was performed via an electrospray ionization source in positive mode. The calibration curve for geniposide was linear (r~2 = 0.997) in the concentration range of 0.005-1 μg/mL. The intra- and inter-day accuracies and precisions fulfilled the required criteria (± 15%). The developed method was subsequently used for pharmacokinetic analysis of geniposide after oral administration to rats at a dose of 50 mg/kg. The mean maximum plasma concentration of geniposide was 0.68 ± 0.29 ug/mL at 0.44 ± 0.13 h, and the mean area under the plasma concentration versus time curve was 1.46 μg-h/mL.