Monitoring of Mixed Culture of Saccharomyces cerevisiae and Acetobacter aceti Using Gravitation Field-flow Fractionation and Gas Chromatography

摘要

Saccaromyces cerevisia (S. cerevisiae) is a species of yast, which is widely used in wine-making and bread-baking-Acetobacter aceti {A. aceti) is used for production of yinegar. Traditionally, vinegar was produced in a mixed culture of yeast and A. aceti The fermentation of S. cerevisiae yields ethanol by an anaerobic process and A. acteti converts ethanol to acetic acid by an aerobic process. Field-flow fractionation (FFF) is a separation technique for macromolecules, and can be useful in various fields including biotechnology. FFF is also well suited to analy-tical-scale separation and characterization of nano to micron-sized particles. FFF has been successfully applied to characterization and separation of colloids with biological interest such as yeast.