Electroacupuncture Could Regulate the NF-rB Signaling Pathway to Ameliorate the Inflammatory Injury in Focal Cerebral Ischemia/Reperfusion Model Rats

摘要

The activated nuclear factor-KappaB signaling pathway plays a critical role in inducing inflammatory injury. It has been reported that electroacupuncture could be an effective anti-inflammatory treatment. We aimed to explore the complex mechanism by which EA inhibits the activation of the NF-kB signal pathway and ameliorate inflammatory injury in the short term; the effects of NEMO Binding Domain peptide for this purpose were compared. Focal cerebral I/R was induced by middle cerebral artery occlusion for 2 hrs. Total 380 male Sprague-Dawley rats are in the study. The neurobehavioral scores, infarction volumes, and the levels of IL-1beta and IL-13 were detected. NF-kappaB p65, IkappaBalpha, IKKalpha, and IKKbeta were analyzed and the ability of NF-kappaB binding DNA was investigated. The EA treatment and the NBD peptide treatment both reduced infarct size, improved neurological scores, and regulated the levels of IL-1beta and IL-13. The treatment reduced the expression of IKKalpha and IKKbeta and altered the expression of NF-kappaB p65 and IkappaBalpha in the cytoplasm and nucleus; the activity of NF-kB was effectively reduced. We conclude that EA treatment might interfere with the process of NF-kB nuclear translocation. And it also could suppress the activity of NF-kB signaling pathway to ameliorate the inflammatory injury after focal cerebral ischemia/reperfusion.