The aromatase inhibitor letrozole reduces adhesion formation after intraperitoneal surgery in a rat uterine horn model

摘要

Objective: To investigate, in an experimental animal study, the effects of letrozole and tamoxifen in the reduction of adhesion formation following abdominopelvic surgery. Study design: Thirty female Wistar albino rats were included and divided into three groups. One group received 500 μg/d tamoxifen and a second group received 1 mg/kg/d letrozole through an enteric tube. A third group did not receive any drugs and served as the control group. On the fifth day, a laparotomy was performed and the right uterine horn was injured by monopolar cautery. The left uterine horn was incised with a scalpel and sutured. The preventive therapy protocols were continued for 7 days after surgery. On the 14th day after first surgery the animals were sacrificed, and the intraperitoneal macroscopic adhesion formation and microscopic adhesion features were evaluated. The Kruskal-Wallis test was used to compare the scores of the macroscopic adhesion scores and histologic features among the three groups, followed by a post hoc Mann-Whitney test. The total histological score was analyzed with a one-way ANOVA, followed by post hoc Bonferroni correction tests. p values ≤0.05 were considered statistically significant. The level of significance was set at p ≤ 0.016 for the post hoc tests. Results: The letrozole and tamoxifen groups had significantly lower adhesion scores for the right uterine horn than the control group (p = 0.005 and p = 0.013, respectively). For the left horn, however, only the letrozole group had a lower macroscopic adhesion score than the controls (p = 0.011). The total histological score was significantly lower in the letrozole group than in the control group (p = 0.014), but no differences were found between the tamoxifen group and the control group (p = 0.954). Inflammation, fibroblastic activity, collagen formation and vascular proliferation were significantly lower in the letrozole group compared with the control group (p < 0.05). The foreign body reactions were similar among the three groups (p > 0.05). Tamoxifen administration did not result in any significant effects on the histological scores (p > 0.05). Conclusion: Letrozole resulted in a significant decrease in postoperative macroscopic adhesion formation and the total histological scores, but tamoxifen did not demonstrate a similar effect on the histological scores.