Cloning and sequence analysis of a hypothalamic cDNA encoding a Dlc dopamine receptor in tilapia

摘要

Physiological and pharmacological studies have indicated that during acid stress a D,-like dopamine receptor becomes functional on intermediate pituitary melanocyte-stimulating hormone cells of tilapia (Oreochromis mossambicus). As a first step towards physiological expression studies we isolated a Drlike dopamine receptor from a tilapia hypothalamus cDNA library. Construction of a phylogenetic tree of most of the D,-like receptors known in human, rat, Xenopws, goldfish and Drosophila revealed that the here presented clone is most likely the tilapia equivalent of the Xenopus Dk dopamine receptor.