Exhaled hydrogen peroxide, nitrite and nitric oxide in healthy children: decrease of hydrogen peroxide by atmospheric nitric oxide.

摘要

Hydrogen peroxide (H subset 2O subset 2) and nitrite (NO subset 2-) in exhaled breath condensate have recently been suggested as non-invasive markers of airway inflammation. The goal of this study was to clarify the role of factors that may potentially influence the measurement of H subset 2O subset 2 and nitrite and to look for possible correlations among these inflammatory markers. - H subset 2O subset 2 and nitrite values were assessed fluorometrically in breath condensate of 102 healthy children (age 4-18 years) and a detailed status of atopy (including history, lung function and skin prick test) was taken in all children. To find out the role of atmospheric nitric oxide, eNO and envNO were measured via chemiluminescence in association with the sampling of the breath condensate. - Median (interquartile range) H subset 2O subset 2 was 0.51 (0.26 - 0.74) mgrg;M and nitrite was 3.3 (2.7 4.1) mgrg;M. A significant negative correlation between H subset 2O subset 2 and envNO was observed (r = -0.50; p < 0.0001). ENO was independent of envNO at our envNO range up to 56 ppb. No further correlation was found. - The inflammatory markers in exhaled breath condensate H subset 2O subset 2, nitrite and eNO are not interrelated to each other in healthy children. Whereas eNO was not dependent on envNO values, high envNO values must be taken into account when measuring H subset 2O subset 2 in exhaled breath condensate.