Automated online solid-phase extraction of albendazole and its metabolites in Chinese mitten crabs followed by stable isotope dilution UPLC-MS/MS analysis.

摘要

The determination of albendazole (ABZ) and its metabolites, albendazole sulfoxide, albendazole sulfone, and albendazole 2-aminosulfone in crab tissue is a complex analysis involving the extraction of trace levels of potentially unstable analytes from a solid matrix. Here, we report on an online solid-phase extraction (SPE) for automated trapping of ABZ and its metabolites in Chinese mitten crab, Eriocheir sinensis H. Milne Edwards (E. sinensis), followed by stable isotope dilution UPLC-MS/MS analysis. The performance of online SPE was fully validated. Good linearity was obtained with correlation coefficients >0.99. In the analytical range, the intra-day and inter-day precision and accuracies (RSD %) were 1.44-4.62 and 3.28-6.93%, respectively. The mean recoveries were in the range of 78.2-109.3%. All these demonstrated that the online SPE is an excellent technique for trapping and purification of ABZ and its metabolites in crab tissue samples. Finally, this online SPE-UPLC-MS/MS strategy was successfully applied to analyze the crude extracts from several crab tissue samples.