Nonaplex real-time PCR detection of Listeria monocytogenes, Campylobacter, Salmonella and enteropathogenic E. coli after universal enrichment in food samples.

摘要

A universal cultivation media for Listeria monocytogenes, Salmonella and enteropathogenic Escherichia coli in conjunction with a highly efficient nonaplex PCR for the determination of L. monocytogenes, Campylobacter, Salmonella and enteropathogenic E. coli was developed and compared to classical microbiological assays. The achieved detection limit was at 2 colony-forming units per g for all analytes. The method allows screening of food samples within 24 h and is partly automatable. A ring trial showed that it can be easily established in other laboratories showing its robustness. The presented method yields results similar to the classical ISO methods based on cultivation