Optimization of culture medium for yellow pigments production with Monascus anka mutant using response surface methodology

摘要

In our laboratory, one Monascus anka mutant able to produce high yield of yellow pigments screened by physical and chemical combination mutagenesis was obtained. This study evaluated peptone, NH4NO3 and KH2PO4 as the most significant variables for Monascus yellow pigment production by this M. anka mutant MYM in submerged fermentation. Response surface methodology (RSM) optimized these nutrient parameters for maximum yellow pigment production (87.24 OD units), which resulted at 10.3 g/L peptone, 11.9 g/L NH4NO3 and 4.7 g/L KH2PO4 in the medium. According to the fitting equation, through five replication experiments under the optimized conditions, the average yellow pigment production obtained was 88.14 OD units for flask cultivation and 92.45 OD units for 5 L fermenter cultivation. Meanwhile, the citrinin could not be detected by HPLC method.