Use of biodiesel-derived crude glycerolfor vancomycin production by Amycolatopsisorientalis XMU-VS01

摘要

Crude glycerol is a primary by-product in the biodiesel industry Microbial fermentation on crude glycerol for producing value-added products provides opportunities to utilize a large quantity of this by-product. This study investigates the potential of using the crude glycerol to produce vancomycin {glycopeptide antibiotics) through fermentation of Amycolatopsis orientalis XMU-VS01. The results show that crude glycerol was the most effective carbon source for mycelium growth and vancomycin production, with 40-60 g/L glycerol concentration as optimal range. Among other culture medium components, potato protein {nitrogen source) and the phosphate concentration had significant effects (p<0.05) for vancomycin production. A Box-Behnken design and response surface methodology were employed to formulate the optimal medium. Their optimal values were determined as 52.73 g/L of glycerol, 17.36 g/L of potato protein, and 0.1 g/L of dipotassium phosphate. A highest vancomycin yield of 7.61 g/L with biomass concentration of 15.8 g/L was obtained after 120 h flask fermentation. The yield of vancomycin was 3.5 times higher than with basic medium. The results suggest that biodiesel-derived crude glycerol is a promising feedstock for production of vancomycin fromA. orientalis culture.