Pattern Recognition of HER-2 in Whole Blood Samples Using Stochastic Microsensors

摘要

Three stochastic microsensors were developed and used for analysis of HER-2 ( a protein from Epidermal Growth Factor Receptor (EGFR) tyrosine kinase family) in whole blood samples. Chitosan I (n = 371 - 744), chitosan II (n = 868 - 1365), and maltodextrin (DE 4-7) were used as modifiers for the design of the new stochastic sensors based on diamond paste. The microsensors proposed can be used for the analysis of HER-2 in the concentration range between 3 fg/mL to 30 ng/mL. The highest sensitivity (1.47 x 10(7) s(-1)/mg/mL) was obtained for the microsensor based on chitosan II and diamond paste, while the lower limit of quantification (3 pg/mL) was obtained when the microsensor based on chitosan I and diamond paste was used. HER- 2 was reliable determined from whole blood samples with recoveries higher than 93.00%.