Cysteamine and beta-mercaptoethanol supplementation improves the in vitro embryo development in buffaloes (Bubalus bubalis)

摘要

The objective of the present experiment was to investigate the effect of cysteamine and beta-mercaptoethanol supplementation on in-vitro maturation, cleavage of oocytes and development of embryo in buffalo (Bubalus bubalis). Oocytes were aspirated from abattoir ovarian follicles of 3-10 mm diameter followed by maturation in the media in vitro containing cysteamine/beta-mercaptoethanol (treatment) and without antioxidant (control). Matured oocytes were co-incubated with sperm (approx. 1x10(6)/ml) of Murrah bull in mSOF medium using heparin (10 mu g/ml). After 22 h of oocyte-sperm incubation, fertilized oocytes were stripped of cumulus cells and cultured in mSOF medium for 8 days to study embryo development. The oocyte maturation rate improved significantly (P<0.05) following addition of 50 or 100 mu M of cysteamine and 10, 50 and 100 mu M of beta-mercaptoethanol (ME), respectively as compared to control. The cleavage rate was found to be significantly (P<0.05) higher at 50 and 100 mu M of cysteamine and at all concentrations of b-mercaptoethanol as compared to control and development of embryos to morula stage was significantly (P<0.05) improved with 50 mu M cysteamine/ beta-mercaptoethanol.