A KIT FOR PARENTAGE VERIFICATION IN BUFFALOES (BUBALUS BUBALIS)

摘要

The present invention relates to a kit for determining the parentage in buffalo (Bubalus bubalis) using microsatellite markers. A core set of 15 microsatellite markers from cattle genome database have been identified and are amplifiable in a single multiplex PCR reaction. Assuming one known parent the core set of markers provide exclusion probabilities of 0.99978 averaged on 13 breeds/ populations of buffaloes from different geographical regions of India. These markers are highly polymorphic, have high heterozygosity, located on different chromosomes and provide a high exclusion probability. The kit has been tested on 12 sires and their 383 daughters belonging to half sib families. The other components of the kit include normal PCR reagents and a standardised protocol.