Use of various methods for detection of sero-group A rotavirus in porcine intestinal and fecal specimens.

摘要

The study was made to find out the etiological agent involved in piglet diarrhoea during 2007-2009 in and around Bareilly. Group A rotavirus (GARV) was found as the main agent using various methods applied to intestinal and fecal samples. Out of 199 necropsied intestinal samples, none was positive in agarose gel electrophoresis, 5 (2.51%) were positive in double antibody sandwich ELISA (DAS-ELISA), 5 (2.51%) in RT-PCR and 18 (9.04%) in real-time RT PCR. Out of 96 fecal samples, 8 (8.33%) were positive in agarose gel electrophoresis, 11 (11.45%) in DAS-ELISA, 16 (16.66%) in RTPCR and 23 (23.95%) were found positive in real-time RT PCR. Thus, it was found that real-time RT PCR, RT-PCR, ELISA and agarose gel electrophoresis have a descending order of sensitivity for detection of rotavirus as has already been reported.