EFFICIENCY OF 1,2 PROPANE DIOL IN CRYOPRESERVATION OF BUFFALO FOLLICULAR OOCYTES

摘要

Oocytes of two distinct qualities those with and without cumulus mass complex were cryopreserved with 2.0M 1,2 Propane diol. After cryopreservation 86.3% A type (oocytes cumulus complex) and 76.0% B type (denuded oocytes) recovered normal. There was significant difference between two groups. The cryopreserved oocytes examined morphologically showed several deformities. Post-thawing oocytes were subjected to maturation process showed maturation rate 15.37% for A type and 13.3% for B type.