The Effect of Bone Morphogenetic Protein 4 on the Differentiation of Mouse Embryonic Stem Cell to Erythroid Lineage in Serum Free and Serum Supplemented Media

摘要

This study was done to compare the effects of bone morphogenetic protein-4 (BMP-4) on mouse embry-onic stem cells (ESC) differentiation to erythroid lineage in serum free and serum supplemented media.The embryoid bodies (EBs) cells were seeded in semisolid serum free and serum supplemented media in thepresence of different concentrations of BMP-4. The erythroid colonies were assessed morphologically, ultra-structurally and by benzidine staining. The expression of the epsilon (e), 3H1 and 13major globins, Runxl and02m genes was evaluated by Real time PCR. The colony size and the percent of benzidine-positive coloniesincreased in both BMP-4 supplementd groups but the number of colonies were lower in these groups thancontrol. Erythropoiesis related genes were expressed in both serum free and serum supplemented groups.There were not significant differences between the ratios of genes expression to Inm in these groups exceptthe ratio of Runxl was significantly higher in serum free group (P<0.05). The ratio of E and 13111 to inm inEBs was higher than both BMP-4 containing groups (P<0.05) and (major was not expressed in EB cells.These findings showed in serum free condition the effects of BMP-4 on the erythroid differentiation wasprominent than serum supplemented group. (Int Biomed Sci 2009; 5(3):275-282)