SPECTROPHOTOMETRY METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF SPIRONOLACTONE AND FUROSEMIDE BY UV-VISIBLE SPECTROPHOTOMETER

摘要

The objective of the current study was to develop a simple, precise and accurate Spectrophometric assay method and validated for determination of Spironolactone and Furosemide in solid pharmaceutical dosage forms. Standard stock solution was prepared in methanol and distilled water in the ratio of 50:50. The emax of Spironolactone at 241 ran and Furosemide at 276 nm were found. The method was validated for specificity, linearity, precision, accuracy, robustness and solution stability. The method was linear in the drug the concentration range of 8-32 μg/ml for Spironolactone and 3.2-12.8ug/ml for furosemide with a correlation coefficient 0.9998 for both Spironolactone and Furosemide. The precision (relative standard deviation-RSD) amongst six-sample preparation for spironolcatone was 0.87% and for Furosemide 1.19% for repeatability and the intermediate precision [RSD] amongst six-sample preparation for Spironolactone was 0.46% and for Furosemide was 0.20%. The accuracy (recovery) for Spironolactone was between 99.94 to 100.05 and for Furosemide 100.00 to 100.04%.