Fluorescence assays for the determination of promoter activities have recently been developed. These assays, however, have been used in a scale too large for high-throughput applications or have been performed under cultivation conditions far from aerobic industrial fermentation processes, because the microorganisms have been either cultivated on agar plates or in multiple-well plates under oxygen-limited process parameters. Such screening conditions could lead to misinterpretation of experimental results. To avoid this, a recently published robotic solution capable of running 768 parallel aerobic cultures in shaken 48-well cell culture plates can be used to automatically apply a fluorescence screening assay. This assay is based on two different fluorescence proteins to monitor the activities of test promoters under aerobic fermentation conditions.
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