Simultaneous Spectrophotometric Estimation of Amoxicillin and Carbocisteine by First Order Derivative Spectroscopy Method in Combined Dosage Form

摘要

The objective of the study was to develop a simple, accurate, precise and rapid UV first order derivative spectrophotometric method. The validation was carried out by using ICH guidelines for the determination of amoxicillin and carbocisteine by using 0.1N hydrochloric acid as the solvent in combined dosage form. The proposed first order derivative method involves the measurement of absorbance of one drug at zero crossing point of other; hence wavelengths 271.2 nm and 219 nm were selected for the estimation of amoxicillin and carbocisteine respectively. The linearity of the proposed method was found in the concentration range of 20 to 100 μg /ml (r~2= 0.9991) for amoxicillin and 10 to 100 ug /ml (r~2= 0.9999) for carbocisteine respectively. The percentage mean recovery was found to be 100.42 % for amoxicillin and 100.156 % for carbocisteine respectively. The method was also statistically validated for its linearity, accuracy and precision. Both intra arid inter day variations showed less percentage (%) RSD values indicating high grade of precision of this method.