Oviduct-specific glycoprotein 1 locus is associated with litter size and weight of ovaries in pigs.

摘要

Oviduct-specific glycoprotein 1 (OVGP1) is implicated in playing a role in fertilization and early embryo development. This study was conducted to determine whether the single nucleotide polymorphism (SNP) of the 9th intron in porcine OVGP1 gene would be associated with female reproductive traits. The sequence of intron 9 of OVGP1 gene in swine was obtained. A total of 273 pigs were used in this study. Comparative sequencing of Meishan (a native Chinese breed) and Large White pig breeds revealed an A/T substitution at position 943. A PCR-EcoRI-RFLP assay was developed to detect this mutation. Polymorphism analysis in Qingping animals showed that pigs with the BB genotype had lower number of piglets born alive (NBA) in multiple parities than pigs with AA (p&0.05) and AB genotypes (p&0.01). In Large White x Meishan (LW x M) F2 offspring, the weight of both ovaries (OW) of the BB genotype was significantly lower than that of AB (p=0.05) and AA (p&0.01) genotypes. Analysis of the data also revealed that the mutation locus affected these 2 traits mostly by additive effects. These studies indicated that the polymorphism was associated with NBA and OW in 2 distinct populations..