Development and Validation of Reversed Phase HPLC Method for the Estimation of Nevirapine in Pure Form and in Pharmaceutical Dosage Forms

摘要

A simple,precise reverse phase high performance liquid chromatogra-phy (RP-HPLC) method was developed for the estimation of nevirapine in bulk as well as in pharmaceutical dosage forms.The quantification was carried out using a BDS Hypersil C-18 column 250 mm x 4.6 mm i.d.,5 mum particle size in isocratic mode,with mobile phase comprising phosphate buffer and acetonitrile in the ratio of 75 : 25 (v/v).The flow rate was 1.5 mL /min and the detection was carried out at 220 nm.The retention time was 6.748 min.The method produced linear response in the concentration range of 60-130 mug/mL and the percentage recovery ranged from 98.15-99.01.