Spectrophotometric Determination of Promethazine Hydrochloride Based on Inhibition of Hemoglobin

摘要

A highly sensitive and simple catalytic spectrophotometric method for the determination of promethazine hydrochloride based on inhibitory effect of promethazine hydrochloride on the hemoglobin-catalyzed the reaction of H2O2 and acid chrome blue K (ACBK) was developed. The concentration of promethazine hydrochloride is linear with the percentage inhibition (I %) of system under the optimal experimental conditions. The calibration graph is linear in range 6.23 x 10~(-8) to 1.56 x 10~(-5) mol L~(-1) with the detection limit of 9.32 x 10~(-10) mol L~(-1). This method can be used for the determination of promethazine hydrochloride in tablets and injection solution of promethazine hydrochloride with satisfactory results.