Interaction of linear mono- and diamines with dimyristoylphosphatidylcholine and dimyristoylphosphatidylglycerol multilamellar liposomes

摘要

The effect of linear monoamines on dimyristoylphosphatidylglycerol and dimyristoylphosphatidylcholine multilamellar liposomes was studied as a function of their length and compared with the behavior of linear carboxylic acids. The role of the hydrophobic interactions was demonstrated and the free energy of the binding for each interacting carbon atom was determined. The thermotropic behavior of the liposomes was characterized by differential scanning calorimetry and it was shown that these molecules affect the temperature and the cooperativity of the gel to fluid state transition of the membrane differently. In particular, it appeared that membrane perturbation was maximum when the chain length of the amphipathic molecules ranged between 7 and 9 carbon atoms, with more pronounced effects in the case of monoamines. Molecules shorter than 3-4 carbon atoms did not produce any observable change in the transition temperature. The study was extended to linear alpha,omega -diamines to investigate the amphipathic character of long diamines and to investigate the role of bridging bonds established with neighboring phospholipids. (C) 2000 Academic Press. [References: 30]