Investigations into the heat stability of bacterial proteases and lipases. II. lipases

摘要

The activity of lipases of 35 strains of bacteria belonging to 8 species (Aeromonas hydrophila, Aeromonas caviae, Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Pseudomonas fluorescens and Serratia marcescens) were tested under 4 time-temperature-combinations and 3 incubation temperatures (2, 7, 30deg C). A modified agar-diffusion test and tributyrin as substrate were used. Loss of activity of all lipases was observed under defined heat treatments (60'/60, 30'/65 and 15'/75deg C). High enzyme activities were also observed at low incubation temperatures for the lipases of Pseudomonas spp., S. epidermidis and Aeromonas hydrophila. It suggested that the data should be taken into consideration when determiningthe keeping quality of heated foods.