A continuous callus culture was obtained from zygotic embryos of Japanese iris (Iris ensata Thunb.) on Murashige-Skoog medium supplemented with 2 mg/l α-naphthylacetic acid and 0.5 mg/l 6-benzy-laminopurine (BAP). It was found that successful callusogenesis required isolated embryos at the wax stage of endosperm development. The optimal combination of phytohormones for the growth of callus tissue was 1 mg/l 2,4-dichlorophenoxyacetic acid and 0.5 mg/l BAP. The pigment composition of I. ensata callus tissue was studied. It was demonstrated that subcultivated callus tissue contained red pigments of flavonoid nature. Under stress cultivation conditions, yellow pigments were formed and the content of red pigments increased.
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机译：在补充有2 mg / lα-萘乙酸和0.5 mg / l 6-苄基-氨基嘌呤（BAP）的Murashige-Skoog培养基上，从日本鸢尾（Iris ensata Thunb。）的合子胚获得连续的愈伤组织培养物。发现成功的愈伤组织形成需要胚乳发育的蜡阶段分离的胚。植物激素对于愈伤组织生长的最佳组合是1 mg / l 2,4-二氯苯氧基乙酸和0.5 mg / l BAP。研究了草愈伤组织的色素组成。已经证明，继代培养的愈伤组织含有类黄酮性质的红色颜料。在胁迫培养条件下，形成黄色颜料，红色颜料的含量增加。
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