Effect of Phytohormones on Plant Regeneration in Callus Culture of Iris ensata Thunb.

摘要

The effect of phytohormones on plant regeneration in callus culture of Iris ensata Thunb. was studied. 2,4-Dichlorophenoxyacetic acid (2,4-D) proved to be the most suitable auxin for the induction and subcul-turing of morphogenic callus. Organogenic calluses were induced from isolated embryos at the waxphase in MS medium supplemented with 2,4-D (1.0-2.0 mg/l) and kinetin (0.2-0.5 mg/l). Changes in the medium hormonal composition favor the development of adventitious structures. The setting of adventitious shoot buds took place in the presence of 6-benzylaminopurine, while the development of shoots and root primordia was observed after 2,4-D replacement with indoleacetic acid (2.0 mg/l). The root initiation in regenerating plants required a hormone-free medium. The development of intact seedling and regenerated plants of 1. ensata were studied. Analysis of the shoot structure demonstrated that in vitro cultured plants are at the juvenile stage.